smooth muscle 22 alpha  (Proteintech)

Journal: Communications Biology

Article Title: Piezo1 promotes vibration-induced vascular smooth muscle injury by regulating the NF-κB/p65 axis

doi: 10.1038/s42003-025-07524-y

Figure Lengend Snippet: a Ca 2+ levels in HUASMC exposed to 0 h, 1 h, 2 h, 3 h, or 4 h vibration were detected by flow cytometry. b Ca 2+ levels are indicated by the mean fluorescence intensity ( n = 3). c Ca 2+ levels in HUASMC measured by flow cytometry of the Control-Static group, Control-Vibration group, GsMTx4-Static group, and GsMTx4-Vibration group. d Ca 2+ levels are indicated by the mean fluorescence intensity ( n = 3). e Representative images of western blot of Piezo1, NF-κB/p65, p53, Caspase 3, sm22α, p16 and GAPDH in the Control-Static group, Control-Vibration group, GsMTx4-Static group, and GsMTx4-Vibration group. f Statistical analysis with results normalized using corresponding internal reference proteins. The experiment was repeated thrice, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. One-way ANOVA is used for analysis.

Article Snippet: PVDF membranes were blocked in TBST containing 5% nonfat skimmed milk and probed for 2 h. Then, PVDF membranes were incubated with primary antibodies against anti-FAM38A/PIEZO1 antibody (rabbit; 1:1000; ab 259949; Abcam), anti-NF-κB p65 antibody (rabbit; 1:1000, ab32536; Abcam), anti-p53 antibody (rabbit 1:1000, ab32049; Abcam), anti-caspase-3 antibody (rabbit; 1:5000; ab32351; Abcam), anti- smooth muscle 22 alpha (SM22)-alpha antibody (rabbit; 1:1000; 10493-1-AP; Proteintech), anti-ARPC5/p16 antibody (rabbit; 1:5000; ab51243; Abcam), or anti-GAPDH antibody (rabbit; 1:10000, 10494-1-AP; Proteintech) overnight at 4 °C.

Techniques: Flow Cytometry, Fluorescence, Control, Western Blot

Journal: Communications Biology

Article Title: Piezo1 promotes vibration-induced vascular smooth muscle injury by regulating the NF-κB/p65 axis

doi: 10.1038/s42003-025-07524-y

Figure Lengend Snippet: a HUASMCs were transfected by si- NC , si-PIEZO1 -001, si-PIEZO1 -002 and si-PIEZO1 -003 after 48 h, Piezo1 was measured by qRT-PCR ( n = 3). b Piezo1 was measured by western blotting after being transfected by si-PIEZO1 -001. c Ca 2+ levels in HUASMC measured by flow cytometry of the NC-Static group, NC-Vibration group, si-PIEZO1 -Static group, and si-PIEZO1 -Vibration group. d Ca 2+ levels are indicated by the mean fluorescence intensity ( n = 3). e Representative images of western blot of Piezo1, NF-κB/p65, p53, Caspase 3, sm22α, p16 and GAPDH in the NC-Static group, NC-Vibration group, si-PIEZO1 -Static group, and si-PIEZO1 -Vibration group. f Statistical analysis with results normalized using corresponding internal reference proteins. The experiment was repeated thrice, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. One-way ANOVA is used for analysis.

Article Snippet: PVDF membranes were blocked in TBST containing 5% nonfat skimmed milk and probed for 2 h. Then, PVDF membranes were incubated with primary antibodies against anti-FAM38A/PIEZO1 antibody (rabbit; 1:1000; ab 259949; Abcam), anti-NF-κB p65 antibody (rabbit; 1:1000, ab32536; Abcam), anti-p53 antibody (rabbit 1:1000, ab32049; Abcam), anti-caspase-3 antibody (rabbit; 1:5000; ab32351; Abcam), anti- smooth muscle 22 alpha (SM22)-alpha antibody (rabbit; 1:1000; 10493-1-AP; Proteintech), anti-ARPC5/p16 antibody (rabbit; 1:5000; ab51243; Abcam), or anti-GAPDH antibody (rabbit; 1:10000, 10494-1-AP; Proteintech) overnight at 4 °C.

Techniques: Transfection, Quantitative RT-PCR, Western Blot, Flow Cytometry, Fluorescence

Journal: Communications Biology

Article Title: Piezo1 promotes vibration-induced vascular smooth muscle injury by regulating the NF-κB/p65 axis

doi: 10.1038/s42003-025-07524-y

Figure Lengend Snippet: a Changes in chemokine levels in culture supernatants of HUASMCs after exposure to 0 h, 1 h, 2 h, 3 h, or 4 h of vibration. b Changes in chemokine levels in cell lysates of HUASMC after exposure to 0 h, 1 h, 2 h, 3 h, or 4 h of vibration. c HUASMCs were transfected by si -NC, si-p65-001 , si- p65-002 and si- p65-003 after 48 h, p65 was measured by qRT-PCR ( n = 3). p65 was measured by western blotting after being transfected by si-p65-001 . d Ca 2+ levels in HUASMCs were measured by flow cytometry of the NC-Static group, NC-Vibration group, si-p65 -Static group, and si-p65 -Vibration group. Ca 2+ levels are indicated by the mean fluorescence intensity ( n = 3). e Representative images of western blot of Piezo1, NF-κB/p65, p53, Caspase 3, sm22α, p16 and GAPDH in the NC-Static group, NC-Vibration group, si-p65-Static group, and si-p65-Vibration group. f Statistical analysis with results normalized using corresponding internal reference proteins. The experiment was repeated thrice, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. One-way ANOVA is used for analysis.

Article Snippet: PVDF membranes were blocked in TBST containing 5% nonfat skimmed milk and probed for 2 h. Then, PVDF membranes were incubated with primary antibodies against anti-FAM38A/PIEZO1 antibody (rabbit; 1:1000; ab 259949; Abcam), anti-NF-κB p65 antibody (rabbit; 1:1000, ab32536; Abcam), anti-p53 antibody (rabbit 1:1000, ab32049; Abcam), anti-caspase-3 antibody (rabbit; 1:5000; ab32351; Abcam), anti- smooth muscle 22 alpha (SM22)-alpha antibody (rabbit; 1:1000; 10493-1-AP; Proteintech), anti-ARPC5/p16 antibody (rabbit; 1:5000; ab51243; Abcam), or anti-GAPDH antibody (rabbit; 1:10000, 10494-1-AP; Proteintech) overnight at 4 °C.

Techniques: Transfection, Quantitative RT-PCR, Western Blot, Flow Cytometry, Fluorescence

Journal: Frontiers in Neurology

Article Title: Establishment and evaluation of a carotid artery dissection model in rats

doi: 10.3389/fneur.2024.1420278

Figure Lengend Snippet: The statistical results of average wall thickness, expression levels of contractile proteins and relative infarction volumes. The average thickness of the walls in group Bt and group Bn were significantly thicker than that in group Wn. There was no significant difference between group Wt and group Wn in average wall thickness (A) . The expression of a-SMA protein in group Bt, group Bn and group Wt were significantly decreased compared with group Wn. The decrease in group Bn and group Wt were not as obvious as that in group Bt (B) . The expression of SM22a protein in group Bt, group Bn and group Wt were significantly decreased compared with group Wn. The decrease in group Bn and group Wt were not as obvious as that in group Bt (C) . The relative infarction volumes of CAD group rats were significantly larger than control group (D) .

Article Snippet: The primary antibodies binding to smooth muscle alpha actin (a-SMA) (Affinity, AF1032) and smooth muscle 22 alpha (SM22a) (Proteintech, 10493-1-AP) should be incubated overnight at 4°C.

Techniques: Expressing, Control

Journal: Frontiers in Neurology

Article Title: Establishment and evaluation of a carotid artery dissection model in rats

doi: 10.3389/fneur.2024.1420278

Figure Lengend Snippet: The expression levels of a-SMA and SM22a contractile proteins in vascular smooth muscle cells of each group. The arrows showed the carotid artery dissections in group Bt.

Article Snippet: The primary antibodies binding to smooth muscle alpha actin (a-SMA) (Affinity, AF1032) and smooth muscle 22 alpha (SM22a) (Proteintech, 10493-1-AP) should be incubated overnight at 4°C.

Techniques: Expressing